Figure 3.

Actin bundling in Arabidopsis is suppressed by the Pst DC3000 cluster IX polymutant. Actin filament architecture in 10-d-old Col-0/GFP-fABD2 cotyledons was quantified at 24 h after dip-inoculation with the Pst DC3000 polymutants. A, CUCPB5459 (ΔI, II, IV, IX, X); B, ΔIX (CUCPB5529); and C, ΔCEL (CUCPB5115). Seedlings were dip-inoculated with Pst DC3000 strains at a concentration of 3 × 10⁷ cfu ml⁻¹ for analyses shown in A and B. In C, seedlings were dip-inoculated with Pst DC3000 at a concentration of 10⁶ cfu ml⁻¹ and with Pst DC3000 ΔCEL at 3 × 10⁷ cfu ml⁻¹. Representative confocal micrographs are shown. Values for actin skewness and density are shown as relative comparisons to Pst DC3000-inoculated plants. Error bars represent mean ± se from the skewness and density values of three independent biological repeats (n = 80–90). Statistical significance was determined using a Student’s t-test, as compared to Pst DC3000 treatment. *, P < 0.01; **, P < 0.001. Bar = 20 μm.