Figure 4.

FoMV-based VIGS of PDS and CLA1 in wheat. A, Wheat plants were infected with a pFoMV-sg vector carrying an inverted-repeat fragment of TaPDS (FoMV-TaPDS) or TaCLA1 (FoMV-TaCLA1). The fourth leaves were photographed at 35 dpi. Bar = 1 cm. B, Quantification of the mRNA levels of TaPDS and TaCLA1 of wheat plants was analyzed by real-time RT-PCR, normalized relative to the level of 18S rRNA. **P < 0.05; ***P < 0.01. Measurements were repeated three times, respectively. The error bar indicates se.