Table I. Summary of starch metabolism genes sequenced in the 187G11 mutant.
Each gene was amplified using primers designed in the untranslated region. PCR products were cloned and sequenced on both strands using additional primers when required. GH and GT stand for glycosylhydrolase and glycosyltransferase, respectively.
| Activity | Gene | CAZy Classification | Accession No. | Sequencing |
|---|---|---|---|---|
| ADP-Glc pyrophosphorylase | glgC | – | KR020055 | + |
| Glycogen/starch synthase | glgA1 | GT5 | AHB52787 | + |
| Glycogen/starch synthase | glgA2 | GT5 | AHB52788 | K480N |
| Glycogen/starch synthase | gbss | GT5 | AHB52786 | + |
| Branching enzyme | glgB1 | GH13 | AFP43334 | + |
| Branching enzyme | glgB2 | GH13 | AFP43335 | + |
| Branching enzyme | glgB3 | GH13 | AFP43336 | + |
| Putative branching enzyme | glgB4 | GH57 | AHB52790 | + |
| Debranching enzyme | glgX1 | GH13 | AGI19288 | + |
| Debranching enzyme | glgX2 | GH13 | AGI19289 | + |
| Debranching enzyme (amylopullulanase GH13) | apu13 | GH13 | AHB52783 | + |
| Putative debranching enzyme (amylopullulanase GH57) | apu57 | GH57 | AHB52784 | + |
| Debranching enzyme (amylo-1,6-glucosidase) | amg | GH133 | AHB52785 | + |
| Phosphorylase | glgP | GT35 | AHB52789 | + |
| α-1,4-Glucanotransferase | malQ | GH77 | AHB52791 | + |