Figure 3.

Mutation in the conserved residues required for CCoAOMT activity did not affect the suppressive role of CCoAOMT2 on Rp1-D21-induced HR. A, Multiple sequence alignment of plant CCoAOMTs. The conserved Arg (R) residue is boxed. B, The HR phenotypes resulting from transient coexpression of CCoAOMT2 and its mutant derivative with Rp1-D21. C, Total protein was extracted from agroinfiltrated leaves at 30 hpi. Anti-HA was used to detect the expression of Rp1-D21, and anti-GFP was used to detect the expression of CCoAOMT homologs or GUS. Equal loading of protein samples was shown by Ponceau S staining.