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. 2016 May 10;171(3):2166–2177. doi: 10.1104/pp.16.00224

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Figure 5. — Investigating the function of CCoAOMT in CC_D21-induced HR. A, CCoAOMT2 but not CCoAOMT1 suppressed CC_D21-induced HR in N. benthamiana. CC_D21:EGFP was transiently coexpressed with EGFP-tagged CCoAOMT1, CCoAOMT2, HCT1806, or GUS, and a representative HR picture was taken at 2 d after inoculation. B, Investigating the interactions between CC_D21 and CCoAOMT and HCT1806 by Co-IP assay. EGFP- and 3×HA-tagged constructs were transiently coexpressed in N. benthamiana, and samples were collected at 30 hpi for Co-IP assay. Protein extracts were immunoprecipitated (IP) by anti-GFP (α-GFP) microbeads and detected (immunblotted [IB]) by anti-GFP and anti-HA (α-HA) antibodies.