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Investigating the interactions between CCD21, HCT and CCoAOMT by Co-IP assay. EGFP-, Myc-, and 3×HA-tagged constructs were transiently coexpressed in N. benthamiana, and samples were collected at 30 hpi for Co-IP assay. Protein extracts were immunoprecipitated (IP) by anti-GFP (α-GFP) microbeads and detected (immunblotted [IB]) by α-GFP, anti-Myc (α-Myc), and anti-HA (α-HA) antibodies.
