Figure 5.

Effect of PMC on PSI photoinhibition induced by rSP treatment. The reaction mixture contains 30 μg ml⁻¹ isolated chloroplasts, and the reaction mixture was maintained at 25°C. A, Time-course analysis of Y(I) in isolated chloroplasts in the absence (black squares) and presence of PMC (5 μm; green stars). Experiments were repeated at least three times, and representative data are shown. B and C, After rSP treatment, the reaction mixture was kept in the dark for 30 min, and the P_m and F_v/F_m values were measured. Data were normalized to the P_m and F_v/F_m values before rSP treatment, and the residual activity of PSI (B) and PSII (C) after rSP treatment were calculated. Data are expressed as mean ± se of three independent experiments.