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. 2016 May 19;171(3):2017–2027. doi: 10.1104/pp.16.00252

Figure 2.

Figure 2.

WRKY46 expression is induced by Fe deficiency. A, Expression of WRKY46 and IRT1 in the wild type with or without Fe deficiency treatment. RNAs were extracted from roots of 6-week-old wild-type plants with or without Fe deficiency treatment for indicated days. Gene expression was determined by RT-qPCR using ACT mRNA as internal reference. B, Expression of WRKY46 in roots via GUS staining without or with Fe deficiency treatment for 2 d. One-week-old transgenic plants carrying a WRKY46pro:GUS construct were used for GUS staining. Bar = 100 μm. C, GUS activity measured in protein extracts from roots (in B) with or without Fe deficiency treatment. Activity units are given nmol methylumbelliferone (μg protein)−1 min−1. Three independent experiments were done. Data were analyzed by one-way ANOVA following Duncan’s test. Error bars with different letters represent a statistical difference (P < 0.05, Duncan’s test).