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WRKY46 regulates the expression of Fe translocation genes. Expression of NAS2 and VITL1 in wild-type and wrky46-1 mutant plants with or without Fe deficiency treatment. RNAs were extracted from roots of 6-week-old wild-type and wrky46-1 mutant plants with or without Fe deficiency treatment for indicated days. Gene expression was determined by RT-qPCR using ACT mRNA as internal reference. Three independent repeats were done with similar results. Error bars indicate sd (n = 3).
