Figure 3.

SlWRKY72b shows Mi-1.2-specific expression following treatment with potato aphid saliva or total protein extract. Leaflets of tomato cv Motelle (Mo) overexpressing SlSERK1-HA, the wild-type cv Motelle, and near isogenic susceptible cv Moneymaker (MM) were infiltrated with potato aphid (PA) saliva collected in vitro in water (A) or PA or GPA protein extracts (B). At 6 h after infiltration, RNA was extracted from leaves and transcript levels were evaluated using RT-qPCR normalized against tomato Actin (SlActin) and calibrated to MM infiltrated with PA saliva or protein extract. Values are average ± se (n = 3). P values were generated by ANOVA using Dunnett’s test for multiple comparisons to MM infiltrated with PA saliva (*P < 0.05, **P < 0.01, and ***P < 0.001). Experiments were performed three times with similar results.