Figure 2.

Unrooted phylogenetic tree of oleosins of Asparagales and Lauraceae species and of Arabidopsis and rice. The four previously recognized lineages of U, SL, SH, and T oleosins as well as the new lineage of M oleosins are highlighted with colors. Oleosins of green algae and primitive plants of the recognized P (primitive) lineage, for simplicity, are not included. For presentation clarity, oleosins of plant species are indicated only with the genus names, which include Arabidopsis (Arabidopsis), Rice (O. sativa), Agapanthus (A. praecox), Agave (A. tequilana), Allium (A. sativum), Scadoxus (S. multiflorus), Lindera (L. glauca), Litsea (L. cubeba), Persea (P. Americana), Phalaenopsis (P. equestris), and Vanilla (V. planifolia). Oleosins of the monocot Elaeis (E. oleifera), with LDs in mesocarp but distant from the Family Lauraceae in phylogeny, are included; Elaeis has no M oleosin. GenBank Accession numbers for the sequences are shown in “Materials and Methods.” The tree was constructed by using the Maximum-Likelihood method with a Jones-Taylor-Thornton substitution matrix supported by a bootstrap test of 1000 resamplings from the aligned oleosin sequences with the PHYLIP package (http://evolution.genetics.washington.edu/phylip/doc/main.html). The scale bar indicates the number of amino-acid substitutions per site. The numbers at the nodes represent the percent bootstrap support.