Figure 4.

AvrRpt2 specifically suppresses the expression of a subset of defense-related genes. DEX-avrRpt2 (rps2 background) and rps2 control plants were treated with 20 µm DEX (5 h) to induce AvrRpt2 expression and expression of the indicated genes tested by qRT-PCR 1 h after water or flg22 infiltration. A, Flg22-inducible genes that are negatively affected by AvrRpt2. B, Flg22-inducible genes that are negatively affected by AvrRpt2 as in A and reported to be regulated by MPK4 and/or MPK11 (Bethke et al., 2012; Frei dit Frey et al., 2014). C, Flg22-inducible genes that are unaffected by AvrRpt2. D, Flg22-inducible MPK3/MPK6-regulated gene, FRK1, positively affected by AvrRpt2. E, MPK genes without known flg22-inducible expression. Note the slightly increased expression upon flg22 treatment in the presence of AvrRpt2. F, Validation of specific avrRpt2 transcript accumulation upon DEX treatment. Letters indicate statistically significant differences (n = 12; one-way ANOVA with Newman-Keuls multiple comparison test after log₂ transformation of the data). nd, Not detectable. Experiments were repeated twice with similar results.