Figure 6.

AvrRpt2 expression enhances infection with bacterial and fungal pathogens. A, DEX-avrRpt2 (rps2 background) and rps2 control plants were treated with DEX (5 h) to induce AvrRpt2 expression, spray inoculated with P. syringae pv tomato DC3000 (5 × 10⁸ bacteria mL⁻¹), and bacterial growth was determined (0 or 3 d post infection [dpi]) by counting colony-forming units (cfu) after plating serial dilutions. The experiment was repeated twice with similar results; the diagram shows combined data sets (n ≥ 20). B, Plants were DEX treated as in A. After 6 h, 10-µL droplets of B. cinerea (strain B05.10) spores (2 × 10⁵ spores mL⁻¹) were inoculated on the leaves and photographed 3 d later. The experiment was repeated twice with similar results. C, Lesion size determination of B. cinerea infection sites of samples described in B. The diagram shows combined data sets (n ≥ 26). D, Fungal biomass in leaves at day 3 was measured by qPCR on the basis of fungal genomic DNA (n = 12). Letters denote statistically significant differences (one-way ANOVA with Tukey’s multiple comparison test, P < 0.001).