Table I. Summary of activities of AvrRpt2 homologs and variants.
Ps, P. syringae pv tomato JL1065; Rs, R. solanacearum CMR15; Ea, E. amylovora ATCC 49946; Ac, A. citrulli strain tw6; Aa, A. avenae ssp. avenae ATCC 19860; Mh, M. huakuii 7653R; Sm, S. medicae WSM1369; Bp, B. pyrrocinia Lyc2; Cf, C. fungivorans; Y, yes; N, no; n.t., not tested.
| Classification | AvrRpt2 Homolog | Percentage Identitya | Autocleavageb | MPK4/MPK11 Inhibition | RIN4 Cleavage | NOI Cleavagec | AXR2/AXR3 Destabilization |
|---|---|---|---|---|---|---|---|
| Phytopathogens | Ps | 100 | Y | Y | Y | Y | Y |
| Rs | 54 | Y | Y | Y | n.t. | n.t. | |
| Ea | 58 | Partial | Y | Y | (Y) | Y | |
| Ac | 49 | Y | Y | Y | n.t. | n.t. | |
| Aa | 27* | ? | N | N | n.t. | n.t. | |
| Plant associated | Mh | 51 | Partial | Y | Y | n.t. | n.t. |
| Sm | 55 | Partial | Y | Y | n.t. | n.t. | |
| Soil/rhizosphere | Bp | 25* | ? | Y | N | (specificNOIs) | Y |
| Fungus infecting | Cf | 61* | ? | N | N | (N) | N |
| P. syringae AvrRpt2 mutants | H208A | 99.6 | N | N | N | (N) | N |
| C122A | 99.6 | N | N | n.t. | n.t. | n.t. | |
| Y191C | 99.6 | Y | Y | Y | (Y) | n.t. | |
| D216E | 99.6 | Y | Y | Y | (Y) | n.t. |
a
Sequence identities (as calculated by Clustal2.1) are based on the complete sequence, resulting in lower scores for truncated sequences, which are marked with asterisks. b?, Autocleavage is unclear, since truncated sequences without cleavage sites were used; partial or incomplete cleavage occurred when multiple bands (including presumed precursor bands) were seen. cInterpretation of NOI cleavage based only on NOI1, NOI7, NOI9, and NOI11 is shown in parentheses.