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Analysis of the products of reactions catalyzed by XXT2 and XXT5 with HPAEC and MALDI-TOF. The products were precipitated (see “Materials and Methods”) and treated with XEG for 12 h. A, HPAEC chromatogram of enzyme assay products and products following XEG digestion. PAD, Pulsed-amperometric detection. B, MALDI-TOF chromatogram of the XXT2 reaction to confirm product sizes. m/z, Mass-to-charge ratio.
