Figure 11.

Localization of PIN1:GFP-F165A by immunogold labeling in roots of seedlings expressing PIN1:GFP-F165A. Labeling with GFP antibodies showed that the PIN1:GFP-F165A mutant accumulates in big structures, from 200 to 500 nm diameter, in stele cells. Lower magnification (top left) shows that these structures (labeled by arrows) are often localized near the plasma membrane. A higher magnification shows that these structures contain multiple membranes and can be seen fusing with the plasma membrane. Arrowheads point to gold particles. G, Golgi. Bars = 500 nm.