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. 2016 May 12;171(3):1965–1982. doi: 10.1104/pp.16.00373

Figure 2.

Figure 2.

Binding of the RBD of Arabidopsis μ-adaptins to different regions of the cytosolic loop of PIN1 and mutant versions. Pull-down experiments used different regions of the cytosolic loop of PIN1 fused with GST (GST-PIN1CL) or mutant versions (Supplemental Fig. S3) and the RBD of Arabidopsis μ-adaptins, with an N-terminal (His)6-tag used for purification (Supplemental Fig. S2). As a control, we used GST. Pull-down results were analyzed by western blotting with His antibodies. The input lane contains 5% of the amount of the μ-adaptin used in the pull-down assay. Arrows point at the expected molecular masses (MW) of the RBD of μ-adaptins A to D. Lower molecular weight bands correspond to the GST-PIN1CL fragments used for the pull-down assays, which cross-reacted with the His antibodies.