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. 2016 May 12;171(3):1965–1982. doi: 10.1104/pp.16.00373

Figure 4.

Figure 4.

Localization and trafficking of PIN1:GFP and different mutant versions of PIN1:GFP at stele cells of Arabidopsis roots. CLSM was performed on primary roots of 4-d-old seedlings expressing PIN1:GFP and different mutant versions of PIN1:GFP in the pin1 background. The left row shows PIN1:GFP-Y260A, PIN1:GFP-Y328A, and PIN1:GFP-Y394A mutants localized to the plasma membrane, like wild-type PIN1:GFP. In contrast, PIN1:GFP-F165A (line 1) showed intracellular protein accumulations that do not appear in roots expressing wild-type PIN1:GFP. This intracellular location also was found for the other PIN1:GFP-F165A lines, either in the pin1 or wild-type background. In the middle row, roots were incubated with 50 μm BFA for 60 min. All lines were able to form BFA compartments, including the PIN1:GFP-F165A mutant. In the right row, BFA was washed out after BFA treatment and roots were analyzed after 90 min. In all lines, most BFA compartments disappeared and fluorescence was visible only at the plasma membrane, except in the PIN1:GFP-F165A mutant, which still contained intracellular punctae similar to those found in the absence of BFA (for details, see text). Bars = 10 μm.