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Immunolocalization of PIN1:GFP-F165A with different organelle markers. Immunolocalization of PIN1:GFP-F165A in primary roots of 4-d-old seedlings was performed as described in “Materials and Methods” with the following organelle markers: ARA7 (PVC), MIN7 (endosomes), Sec21 (Golgi), plasma membrane (PM) ATPase, E2 (EXPO compartments), and P2 (SH3P2). Bars = 10 μm.
