Figure 2.

Analysis of SAA and ROS-responsive transcripts after localized endogenous chloroplastic ¹O₂ generation within a single leaf. A, The dark-light shift treatment method of a single leaf using the conditional flu mutant. Leaf covers were applied to leaf position 6 for 12 h in order for protochlorophyllide (Pchl) to accumulate in the dark. Leaf covers were then removed for 1 h under growth light (120 μmol photons m⁻² s⁻¹) to generate localized endogenous ¹O₂. Pchl, Protochlorophyllide. B, RRTF1 (SAA marker), ZAT10 (SAA marker), ELIP2 (HL marker), NodL (¹O₂ responsive SAA marker), AAA-ATPase (SAA-independent ¹O₂ marker), and FER1 (H₂O₂ marker) transcript accumulation in treated and distal tissues of the flu mutant relative to untreated flu plants. LL, low light; ¹O₂, endogenous ¹O₂ treated leaf position 6; D1, lower distal leaf position 5; D2, upper distal leaf position 7. One-way ANOVAs were performed on each data set, post hoc Fisher's lsd results are displayed as a bar in the top left corner of each plot. n.s., Not significant. Error bars represent se, n = 3.