Figure 5.

The bps signal is sufficient to reduce cytokinin responses. A, Shoot expression of the CK response marker pARR5::GUS in 5-d wild-type (Col-0) and bps1-2, mock-treated or transferred to 0.01 to 10 µM CK (BAP) for 24 h. Bars = 50 µm. B, Transient graft assay of pARR5::GUS in 5-d wild-type plants that were intact or scions that were coupled to wild-type or bps1 roots. GUS staining was carried out 24 h after transient graft establishment. Bars = 50 µm. C, Quantitative analysis of AHK and AHP expression in 5-d wild-type shoots transiently grafted to either a wild-type (Col-0) or bps1-2 root. Data are shown as the mean ± sd of three biological and two technical replicates.