Figure 5.

Complementation of the vipp1 knockout mutant (vipp-ko) with Pv-VIPP1-GFP or Pv-VIPP1Δc-GFP, and characterization of heat shock tolerance. A, Schematic illustration of two constructs used for complementation: Pv-VIPP1-GFP and Pv-VIPP1Δc-GFP. B, Photographs of 10-d-old seedlings from different lines (upper panels, bar = 3 mm) and chloroplast/plastid observed directly using bright-light microscopy (lower panels; bars = 10 μm). Colorless plastid of vipp-ko mutant is indicated by a red arrowhead. C, Accumulation of VIPP1-GFP and VIPP1Δc-GFP confirmed by western blots. D, In situ observation of VIPP1-GFP and VIPP1Δc-GFP particles in the leaves of Pv-VIPP1-GFP/vipp1-ko and Pv-VIPP1Δc-GFP/vipp1-ko, respectively. E, The Fv/Fm (left) and Rfd (right) of detached leaves from 3-week-old seedlings of Col (blue), Pv-VIPP1-GFP/vipp1-ko (red), and Pv-VIPP1Δc-GFP/vipp1-ko (green) that were treated with a heat shock at 45°C for 6 min.