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. 2016 May 2;171(3):2127–2139. doi: 10.1104/pp.16.00540

Figure 5.

Figure 5.

NPY1p regulates the peroxisomal NADH homeostasis in the mdh3Δ mutant. A, Constitutive expression of PXN (pHHU274; 36.1 kD) in the npy1Δ, mdh3Δ, and mdh3/npy1Δ mutant was confirmed by immunoblot analysis using PXN-specific antibody. Arrowhead indicates the detected protein band for the recombinant PXN. Lane 1, Wild-type cells; lane 2, npy1Δ cells; lane 3, npy1Δ cells expressing PXN; lane 4, mdh3Δ cells; lane 5, mdh3Δ cells expressing PXN; lane 6, mdh3/npy1Δ cells; lane 7, npy1Δ cells expressing PXN; lane 8, fox1Δ cells. B, Octanoic acid β-oxidation activity in oleate-induced yeast wild-type and mutant cells. The strains shown are as follows: wild type, mdh3Δ, npy1Δ, and mdh3/npy1Δ transformed with empty vector or expressing PXN. Relative FAO rates were calculated, in which the activities in wild-type cells were taken as reference (100%). Data are represented as arithmetic means ± sd of technical triplicates. Asterisks indicate statistical differences to the wild type with *** P < 0.001 as very significant and *P < 0.05 as significant. Hash tags indicate statistical differences to mdh3Δ cells with ###P < 0.001 as very significant. Large dots indicate statistical differences between mdh3Δ and mdh3/npy1Δ expressing PXN with ▪▪▪P < 0.001 as very significant.