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. 2016 May 15;171(3):1794–1800. doi: 10.1104/pp.16.00663

Figure 1.

Figure 1.

Design of a CRISPR/Cas9 vector to facilitate a visual screen for Cas9-free Arabidopsis seeds in the T2 generation. A, Schematic representation of the new CRISPR/Cas9 vector that contains a Cas9 expression cassette driven by the cauliflower mosaic virus (CaMV) 35S promoter and a U6 promoter-controlled gRNA production unit. More importantly, it also expresses mCherry from the strong promoter At2S3 in seeds. NLS, Nuclear localization signal. B, Visual screen for T2 seeds that no longer harbor the CRISPR/Cas9 construct. The Cas9-free seeds do not produce the red fluorescence.