Table I. Editing efficiencies by CRISPR/Cas9 for different target sites.

The target sequences for CRP2 and CRP3 are described in Figure 2A. The RGR target site was described previously (Gao et al., 2015). The RGR sequence and design also are shown in Supplemental Figure S2. CRP2/RGR refers to targeting both CRP2 and RGR sites simultaneously. The ratios represent the editing efficiency. For example, 7:33 refers to seven positive plants out of 33 total plants. All of the T2 plants analyzed were Cas9 free based on a lack of red fluorescence (Fig. 1B). For the T2 plants in boldface, the number of mutant plants (both heterozygous and homozygous) from each T1 plant is shown (boldfaced indicates non-bi-allelic mutations). abp1-c12d and abp1-c42d are from the same T1 plant 75; abp1-c2 shows an unusual segregation pattern.

Target	CRP2	CRP3	CRP2/RGR	CRP3/RGR
T1	7:33	3:86	5:61	0:92
T2	T1 plant 3, died	T1 plant 34, 0:72	T1 plant 14, 0:48	Not analyzed
	T1 plant 5, 0:72	T1 plant 40, 0:72	T1 plant 29, 26:52 (abp1-c2)	Not analyzed
	T1 plant 11, 1:95		abp1-c2+/−, 13:52	Not analyzed
	T1 plant 14, 3:94	T1 plant 75, 8:196	abp1-c2−/−, 13:52	Not analyzed
	T1 plant 25, 1:95	abp1-c12d, 7:196	T1 plant 38, 0:72	Not analyzed
	T1 plant 30, 0:72	abp1-c42d, 1:196	T1 plant 56, 2:96 (abp1-c3)	Not analyzed
	T1 plant 33, 0:96		T1 plant 65, 0:72	Not analyzed