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. 2016 May 31;171(3):1951–1964. doi: 10.1104/pp.16.00664

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Figure 5. — Expression profiles of fatty acid biosynthetic genes in transgenic Arabidopsis plants with different MED15 and WRI1 transcript levels. A, Expression levels of BCCP2, FAD2, and KAS3 in wild-type Col-0, wri1-4, MED15Pro::MED15-Ri, WRI1-OE-2, MED15-OE-2, WRI1-OE-1/MED15Pro::MED15-Ri, and MED15-OE-1/wri1-4 transgenic plants. B, Expression levels ACP1, FAD3, KAS1, SUS2, Ch-PKα, Cy-PKβ, and FAE1 in wild-type Col-0, wri1-4, MED15Pro::MED15-Ri, WRI1-OE-2, MED15-OE-2, WRI1-OE-1/MED15Pro::MED15-Ri, and MED15-OE-1/wri1-4 transgenic plants. C, Expression levels of OLE1 in wild-type Col-0, wri1-4, MED15Pro::MED15-Ri, WRI1-OE-2, MED15-OE-2, WRI1-OE-1/MED15Pro::MED15-Ri, and MED15-OE-1/wri1-4 transgenic plants. Two-week-old Arabidopsis seedlings were used for qRT-PCR analysis. The expression values were normalized using ACTIN1 as an internal control. Each experiment was repeated with three biological replicates. Student’s t test: *, P < 0.05, **, P < 0.01, or ***, P < 0.001 versus wild-type Col-0; ^a, P < 0.05, ^aa, P < 0.01, or ^aaa, P < 0.001 versus wri1-4; ^b, P < 0.05, ^bb, P < 0.01, or ^bbb, P < 0.001 versus MED15Pro::MED15-Ri. Error bars represent sd.