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. 2016 Jul 7;12(7):e1005747. doi: 10.1371/journal.ppat.1005747

Fig 6. Altered functions of KLF4 in HPV-positive keratinocytes.

Fig 6

(6A). Genetically matched sets of HFK and HFK-31gen cells were transiently silenced for KLF4 using lentiviral transduction and were subsequently analyzed for their ability to grow as organotypic rafts. KLF4 silenced HFK-31gen cells grew as rafts with morphologically altered cornified envelope, whereas KLF4 silenced HFKs did not form rafts. (6B). KLF4 silenced HFKs did not form any colonies in holoclone assay compared to controls, whereas KLF4 silenced HFK-31gen cells formed colonies comparable to controls. (6C. i) KLF4 is stably silenced in HFK-31gen cells. KLF4 protein levels were reduced in both undifferentiated and differentiated conditions compared to controls as shown in the western blot. (ii) Stable KLF4 silenced HFK-31gen cells formed rafts with morphologically altered cornified envelope. (6D) Raft sections were analyzed by immunofluorescence for KLF4, loricrin, and E1^E4. KLF4 silenced HFK-31gen rafts showed reduced KLF4 and no loricrin or E1^E4 staining compared to controls.