Table 1. PCR and IHC Detection of M. avium subsp. paratuberculosis in uninfected terminal small intestine segments (IPP-C), adjacent infected segments (IPP-M), and mesenteric lymph nodes (LN).
| Calf | Infection (Mon) | PCR1 | IHC2 | |||
|---|---|---|---|---|---|---|
| IPP-C3 | IPP-M3 | IPP-C | IPP-M | LN | ||
| 4 | 2.5 | – | – | + | + | + |
| 5 | 2 | + | + | + Tiss | + Tiss | + |
| 6 | 2 | – | + | – | + | – |
| 7 | 2 | – | + | – | + | – |
| 8 | 2 | – | + | + | + | – |
| 9 | 1 | + | + | + | + | + |
| 10 | 1 | – | + | – | + Cont | + |
1PCR amplification using VetAlert™ Johne’s Real-Time PCR
2 M. avium subsp. paratuberculosis was detected using rabbit polyclonal antisera that may cross-react with other mycobacterial
3Continuous PP and draining MLN tissue were combined on the same slide used for the PCR reaction
Tiss = Tissue section with no visible intestinal contents attached to the mucosa
Cont = Intestinal contents on the slide without intestinal tissue