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. 2016 Jul 7;11(7):e0157670. doi: 10.1371/journal.pone.0157670

Fig 2. Heterogeneity of mouse-derived stroma in breast cancer tumors.

Fig 2

(a) Flow cytometry analysis of tumor-infiltrating hematopoietic stromal cells after dissociation of the HBCx-9 tumor graft and staining with anti-EpCAM, CD45, F4/80, Ly-6G, Ly-6C and CD19 antibodies. Viable murine macrophage-like cells (DAPI-EpCAM-CD45+CD11b+F4/80+), monocytes (DAPI-EpCAM-CD45+CD11b+F4/80+Ly-6Chi), granulocytes (EpCAM-CD45+CD11b+Ly-6G+) and B lymphocytes (EpCAM-CD45+CD11b-CD19+) were identified as separate populations. (b) Flow cytometry analysis of individual leukocyte populations as a percent of total CD45+ cells in MMTV-PyMT/BC-PyMT and MMTV-Erbb2/BC-Erbb2 mammary tumors and in 20 PDX. The data shown are the mean percentages of viable cells ± SEM (standard error of the mean) for three mice per cohort. BC models were compared in Kruskal-Wallis tests. (c) Comparison of dissociated cell profiles showing the similarity of spontaneous and allografted MMTV-PyMT/BC-PyMT and MMTV-Erbb2/BC-Erbb2 tumors on the basis of anti-EpCAM, CD45, Ly-6C and F4/80 staining. (d) Specific stromal profiles of BC tumors. Clustering of stromal population percentage data from flow cytometry shown in (2b) above.