Table 1. Fold Changes in the Genes Regulated by p21 Deletion in the FLT3-ITD-transformed KSL Cells.

Gene symbol	Name	Fold change after p21 deletion in FLT3-ITD+ KSL cells	Number of functional classification hits (as shown in Fig 7A)
Pbx1	Pre-B cell leukemia transcription factor 1	2.1	7
Vegfa	Vascular endothelial growth factor A	1.8	4
Trib1	Tribbles homolog 1 (Drosophila)	2.3	4
Skil	SKI-like	1.6	3
Tcf4	Transcription factor 4	2.1	2
Rasgrp1	RAS guanyl-releasing protein 1	-2.5	1
Hdgfrp3	Hepatoma-derived growth factor-related protein 3	1.7	1

P21 deletion in FLT3-ITD⁺ KSL cells resulted in the modulation of the expression levels of 111 mRNAs that were either unaffected or differentially regulated in normal bone marrow KSL cells. Of these 111 genes, 12 were deregulated in human AML-LSC [40]. These genes were classified based on Gene Ontology terms and the Swiss Protein database (as shown in Fig 7B). Pbx1 was classified into 7 of the functional groups. The fold changes in the expression levels of the genes listed in Fig 7B are shown in Table 1.