Table 1. Primers used for PCR and sequencing.
| Gene | Oligo Sequence (5'-3') | Amplicon size |
|---|---|---|
| gltA | 1,2CS49Fw: ACCTATACTTAAAGCAAGTATYGGT | 385bp |
| 1CS1234Rv: TCTAGGTCTGCTGATTTTTTGTTCA | ||
| 2CS1258Rv: ATTGCAAAAAGTACAGTGAACA | ||
| ompB | 1,2RAK1009Fw: ACATKGTTATACARAGTGYTAATGC | 444bp |
| 1OmpB1902Rv: CCGTCATTTCCAATAACTAACTC | ||
| 2RAK1452Rv: SGTTAACTTKACCGYTTATAACTGT | ||
| ompA | 1OmpAM50Fw: TTGCGTTATAACACTTTTTAAGTGA | 530bp |
| 1OmpA642Rv: ATTACCTATTGTTCCGTTAATGGCA | ||
| 2190-70Fw: ATGGCGAATATTTCTCCAAAA | ||
| 2190-701Rv: GTTCCGTTAATGGCAGCATCT | ||
| sca4 | 1D1Fw: ATGAGTAAAGACGGTAACCT | 2700bp |
| 1D3069Rv: TCAGCGTTGTGGAGGGGAAG | ||
| 2RrD749Fw: TGGTAGCATTAAAAGCTG | ||
| 2RrD2685Rv: TTCAGTAGAAGATTTAGT | ||
| 17kDa | 1Rp17kFw: AATGAGTTTTATACTTTACAAAATTCTAAAAACCA | 450bp |
| 1,2Rr2608Rv: CATTGTTCGTCAGGTTGGCG | ||
| 2Rr1175Fw: GCTCTTGCAACTTCTATGTT |
Fw = Foward primer; Rv = Reverse primer;
1 = primary PCR;
2 = secondary PCR and sequencing