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. 2016 Jul 7;11(7):e0157907. doi: 10.1371/journal.pone.0157907

Fig 1. IL-2R subunits are present in differentiated murine podocytes.

Fig 1

(A) Representative flow cytometry histograms showing surface IL-2R subunits α and γ (black and green histograms). Nonspecific staining (black histogram) was determined using an isotype-specific control antibody. Murine podocytes express the three subunits of the IL-2R. (B) IL-2R α alpha and β chains are expressed in non-stimulated podocytes. Differentiated podocytes express α alpha and β mRNA subunits of the IL-2R as shown by real time PCR. (C) The alpha subunit is demonstrated by Western blot. The positive control consists of protein extracts obtained from Jurkat (JKT) cells. (D) The conditionally immortalized murine podocyte clone JR07 expresses podocyte specific proteins nephrin and podocin in proliferating and differentiating cells as demonstrated by Western blot. (E) Whole cell lysates of murine podocytes were immunoblotted with anti- alpha, beta, and gamma chains antibodies showing the presence of the whole complex of the IL-2R by Western blot. (F) IL-2R expression in podocytes from human renal biopsies. Immunohistochemical staining was performed on human renal biopsies from children with FSGS and control. CD25 (alpha chain) expression was observed in biopsies from children with FSGS on the surface podocytes, indicated by the arrows (left panel) when compared to the biopsy control (right panel).