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. 2016 Jul 7;11(7):e0158597. doi: 10.1371/journal.pone.0158597

Fig 5. GnRH-E1 RNA, Gnrh1 mRNA, and Gnrh1 pre-mRNA stability following actinomycin D treatment of GT1-7 neurons.

Fig 5

GT1-7 neurons were treated with either DMSO control vehicle (black bars) or 1 μg/mL actinomycin D (white bars). Total RNA was harvest at 2, 4, 8, and 24 hours after treatment. RT-qPCR analysis was performed to determine changes in endogenous mouse GnRH-E1 RNA (A), transgene-derived rat GnRH-E1 RNA (B), Gnrh1 pre-mRNA (C) and Gnrh1 mRNA (D) expression. Relative expression is normalized to H2afz mRNA control. Data are displayed as the fold change from untreated cells that were harvested at the time of treatment, and as the mean ± SD. Statistical significance was determined by two-way ANOVA, followed by post hoc Tukey-Kramer HSD, where asterisks indicate statistical significance at p<0.05.