Table 1. Oligonucleotide sequences.
| Primer Name | * | Sequence (5’ to 3’) | Figures |
|---|---|---|---|
| Mouse GnRH-E1 RNA -3371 | F | GGGAAAGGAAAGCAATTTCA | 3C-D |
| Mouse GnRH-E1 RNA -3560 | F | AGCTGTGTCCAAATGGGTTC | 3A-D; 7A and D |
| Mouse GnRH-E1 RNA -3606 | F | CGTAGGTGTCCCAGTGTCCTTATTTGTTGC | 3A-B |
| Mouse GnRH-E1 RNA -3746 | F | CTCTATGTCAGTACCTGTGGCTCGGGCTCG | 3A-B |
| Mouse GnRH-E1 RNA -3779 | F | TGAACAAAGGAAGCATTTGG | 3A-B |
| Mouse GnRH-E1 RNA -1271 | R | GCAGATGCTGCCTCTATTTCA | 3A-D |
| Mouse GnRH-E1 RNA -1128 | R | GCCTGCCTCTGAAACTTTTG | 3A-D |
| Mouse GnRH-E1 RNA -1443 | R | TGCTAACCCCAATCCGCCATTGCTT | 3C-D |
| Mouse GnRH-E1 RNA -2438 | R | GCGTCAAACTACAAAGGAAAGTGGCTGCAT | 2A and C; 5A; 6A |
| Mouse GnRH-E1 RNA -2106 | R | TAGGCCAATTTACCCAAGACCTGAAATTGA | 2A and C; 5A; 6A |
| Rat GnRH-E1 RNA -1640 | F | GCAGACAGAGGAGCTGAGAGATGCAAAC | 2B and C; 5B; 6B |
| Rat GnRH-E1 RNA -1385 | R | AGGGTAGGGAAGAGGGACTTTGTGGTG | 2B and C; 5B; 6B |
| Mouse Gnrh1 mRNA +1144 (exon) | F | CTACTGCTGACTGTGTGTTTG | 5C-D; 6C-D |
| Mouse Gnrh1 mRNA +4059 (exon) | R | CATCTTCTTCTGCCTGGCTTC | 5C; 6C |
| Gnrh1 pre-mRNA +1297 (intron) | R | GCTCATGCAGTTTGAAGCGAGTC | 5D; 6D |
| Mouse H2afz mRNA | F | TCACCGCAGAGGTACTTGAG | 5 and 6 |
| Mouse H2afz mRNA | R | GATGTGTGGGATGACACCA | |
| Mouse Ppia mRNA | F | AAGTTCCAAAGACAGCAGAAAAC | 2C |
| Mouse Ppia mRNA | R | CTCAAATTTCTCTCCGTAGATGG | 2C |
| Mouse GnRH-E1 RNA siRNA sense | AAACUACAAAGGAAAGUGG | 6 | |
| Mouse GnRH-E1 RNA siRNA antisense | CCACUUUCCUUUGUAGUUU | 6 | |
| siRNA negative control pool | UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, UGGUUUACAUGUUUUCCUA | 6 |
* Indicates primer direction of forward (F) or reverse (R).