Fig 3. Distant organ injuries in mice with polymicrobial sepsis.

(A) Mice were subjected to CLP procedures to induce polymicrobial sepsis. At 24 h post-CLP, lung, kidney, liver and spleen tissues were sectioned for H&E staining and the lung was immunostained with Ly6G or F4/80. (B) MPO determined in homogenates from above-indicated tissues. (C) 24 h after CLP, neutrophils were isolated from blood for H₂DCF and MPO assays after 1 h incubation. (D) CFUs evaluated with the lung and kidney as above (n = 3, means±SD). A, data are representative of three independent experiments. B, C, means+SD from triplicate. One-way ANOVA (Tukey’s post hoc); *, p<0.05. Scale bar = 5 μm.