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. 2016 Jul 7;12(7):e1005743. doi: 10.1371/journal.ppat.1005743

Fig 5. Neutrophils and monocytes are main sources of IL-17A upon CLP.

Fig 5

(A) anxa2 -/- mice were subjected to CLP procedures to induce polymicrobial sepsis. At 24 h post-CLP, colon tissues were sectioned for immunostaining with RORγt, NK1.1, F4/80, Ly6G, and IL-17A antibodies. (B) Colocalization of red fluorescence (to label indicated cell types) with green fluorescence (IL-17A+) quantified as percentage of total green fluorescence. (C) IL-17A positive events in peritoneal lavages from anxa2 -/- mice at 24 h post-CLP. The IL-17A+ cells were collected and further stained with different antibodies and shown as scattergrams. Data are representative from 3 independent experiments. Scale bar = 100 μm.