Fig. 1.

Microbial stimuli promote translocation of Mincle and MCL to the cell surface. BMM were treated for 16 h (h) with various microbial stimuli, as indicated, and surface (A–C) and intracellular (D) protein expression assessed for MCL or Mincle, by flow cytometry as indicated. MCL total cellular protein was also analysed over time by Western blot analysis (E). Data shown from individual experiments in duplicate and are representative of two independent experiments. Data shown as mean + standard deviation (SD). *, p < 0.05 compared to untreated control of the same strain. #, p < 0.05 compared to the same treatment of the wild-type strain. –, unstimulated control.