Skip to main content
. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2016 May 20;15(7):1691–1701. doi: 10.1158/1535-7163.MCT-16-0032

Figure 4. Transfection of CDK11 siRNA into ovarian cancer cells induces apoptosis.

Figure 4

A: Transfection of CDK11 siRNA into ovarian cancer cells decreased cell proliferation and inhibited cell growth in a time-dependent manner. SKOV-3 and OVCAR-8 cells were transfected with CDK11 siRNA (20 nM). Proliferation of cancer cells were assessed at different time points post transfection by MTT as described in the Materials and Methods. B: Knockdown CDK11 induced apoptosis in ovarian cancer cells. SKOV-3 and OVCAR-8 cells were transfected with CDK11 siRNA (20 nM). Total cellular proteins were extracted 48 hours post transfection and were subjected to immunoblotting with specific antibodies to PARP and β-actin as described in Materials and Methods. Knockdown of CDK11 led to increased expression of cleaved PARP. C: Apoptosis was also evaluated by the Apo-ONE Homogeneous Caspase-3/7 Assay kit as described in Materials and Methods.