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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Pharm. 2016 Jan 11;13(2):357–368. doi: 10.1021/acs.molpharmaceut.5b00542

Figure 2.

Figure 2

Fluid-phase uptake via clathrin- or caveolae-mediated endocytosis in LSD fibroblasts. (A) Quantification of the percent colocalization of Texas Red dextran-positive vesicles internalized by wild-type fibroblasts (30 min, 37°C) along with green fluorescent transferrin (Tf) or cholera toxin B (CTB). Colocalizing area and number of vesicles are shown. (B, C) Quantification of the number of Texas Red dextran-positive vesicles internalized during 1 h at 37°C by wild-type vs. diseased cells incubated with: (B) monodansylcadaverine (MDC), an inhibitor of clathrin-mediated endocytosis, or (C) filipin, an inhibitor of caveolar endocytosis. Data are normalized to those of cells incubated in the absence of inhibitors (control (Ctr)), which is indicated by the horizontal line. The difference between the control line and the inhibitor bar demonstrates the relative contribution of each pathway to fluid-phase uptake. Data are the mean ± SEM. *Comparison with uninhibited, control cells (p<0.05 by Student’s t-test).