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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Pharm. 2016 Jan 11;13(2):357–368. doi: 10.1021/acs.molpharmaceut.5b00542

Figure 4.

Figure 4

Transferrin endocytosis in LSD fibroblasts. Wild-type (Wt), NPD, NPC, Gaucher, and Fabry fibroblasts incubated with fluorescent transferrin (Tf; green pseudocolor) for 1 h at 37°C. (A) Fluorescence images were taken after unbound Tf was washed off and surface-bound Tf was immunostained (red pseudocolor). This rendered surface-bound Tf marked in two colors (red + green = yellow; arrowheads), distinguishable from internalized Tf (green; arrows). NPD, reported in 30 serves as a control and is not shown. Dashed lines = cell borders, as observed from phase-contrast microscopy. Scale bar = 10 μm. (B) Quantification of the percentage of single-labeled Tf found inside each cell compared to total (single-labeled and double-labeled) Tf associated with said cells. (C) Internal and surface Tf (fluorescent objects over background). (B,C) Data are the mean ± SEM. *Comparison with Wt (p<0.05 by Student’s t-test).