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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2016 May 9;15(7):1485–1494. doi: 10.1158/1535-7163.MCT-15-0566

Figure 5. GS87-induced AML differentiation is dependent on MAPK signaling.

Figure 5

(A) Comparison of GSK3 inhibitor modulation of gene expression in AML cells. HL-60 cells were treated for 48 hours and the expression of the indicated genes was assessed. Left Panel: Venn diagram of significantly regulated genes. Right Panel: Validation of microarray results by real-time PCR. (B) Multiple components of the MAPK signaling cascade are activated by GS87 treatment. HL-60 cells were treated with GS87, Li, or SB and the activation of p38, ERK, and JNK were analyzed by Western blot at various time points using phospho-specific antibodies. (C) Chemical inhibition of ERK (PD08959 10μM) abrogates the differentiation capacity of GS87 in HL-60 cells. **p<.01.