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. 2016 Jun 19;9(6):605–610. doi: 10.14202/vetworld.2016.605-610

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Copyright: © Kusindarta, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-2 — Human umbilical mesenchymal stem cells conditioned medium (HU-MSCM) promote angiogenesis. Vascular endothelial growth factor (VEGF) (white bar), monoclonal antibodies clone LM609 (gray bar), in concentration 40 µg/ml and HU-MSCM (black bar) was added to endothelial cells, and tube formation was investigated by microscopy. Data are given as mean of tube length in µm + standard deviation (upper panel). Statistical analysis was performed by one-way ANOVA followed by Bonferroni’s post-hoc test; n.s. = Not significant, VEGF = Control positive tube formation, LM609 = Control negative tube formation. Representative microphotographs of endothelial tube formation assays as outlined are given in the lower panel.