Table 4.
Bacterial groups detected by fluorescence in situ hybridisation in the pH-controlled and volume-controlled batch cultures at 0, 4, 8 and 24 h of fermentation* (Mean of the data of three experiments and standard deviations; n 3)
| Bacterial population (log10 cells/ml) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 52 % B-GOS 1 g | 52 % B-GOS 0·5 g | 52 % B-GOS 0·33 g | 65 % B-GOS 1 g | 65% B-GOS 0·5 g | 65 % B-GOS 0·33 g | NC | |||||||||
| Probes | Time point (h) | Mean | sd | Mean | sd | Mean | sd | Mean | sd | Mean | sd | Mean | sd | Mean | sd |
| Eub338 I-II-III | 0 | 9·24 | 0·09 | 9·13 | 0·11 | 9·22 | 0·11 | 9·24 | 0·11 | 9·27 | 0·12 | 9·23 | 0·1 | 9·19 | 0·08 |
| 4 | 9·11 | 0·17 | 9·15 | 0·18 | 9·14 | 0·44 | 9·39 | 0·14 | 8·9 | 0·14 | 9·35 | 0·07 | 9·04 | 0·31 | |
| 8 | 9·16 | 0·13 | 9·49 | 0·23 | 9·05 | 0·41 | 9·52 | 0·1 | 8·92 | 0·1 | 9·46 | 0·04 | 9·2 | 0·59 | |
| 24 | 9·14 | 0·01 | 9·33 | 0·03 | 9·1 | 0·05 | 8·94a,c | 0·07 | 9·21 | 0·01 | 9·56a | 0·04 | 8·95a | 0·02 | |
| Lab158 | 0 | 7·63 | 0·09 | 7·48 | 0·11 | 7·55 | 0·05 | 7·52 | 0·07 | 7·54 | 0·15 | 7·47 | 0·06 | 7·48 | 0·06 |
| 4 | 7·57 | 0·05 | 7·64 | 0·16 | 7·77 | 0·27 | 7·78 | 0·14 | 7·73 | 0·12 | 7·31 | 0·29 | 7·54 | 0·2 | |
| 8 | 7·74 | 0·1 | 8·05 | 0·11 | 7·6 | 0·36 | 7·98 | 0·27 | 8·21 | 0·56 | 7·66 | 0·12 | 7·55 | 0·4 | |
| 24 | 8·39a,b,c | 0·02 | 7·70c | 0·01 | 7·94a | 0·07 | 7·94a | 0·09 | 7·68 | 0·06 | 7·85a | 0·05 | 7·29a | 0·08 | |
| Bif164 | 0 | 8·61 | 0·28 | 8·7 | 0·17 | 8·58 | 0·26 | 8·45 | 0·35 | 8·63 | 0·21 | 8·47 | 0·3 | 8·68 | 0·21 |
| 4 | 8·73 | 0·33 | 9·1 | 0·22 | 8·79 | 0·67 | 8·86b | 0·2 | 8·75 | 0·19 | 8·47 | 0·4 | 8·45 | 0·31 | |
| 8 | 8·93 | 0·29 | 8·87 | 0·61 | 8·95 | 0·63 | 9·12 | 0·21 | 9·25a,b | 0·04 | 9·31a,b | 0·1 | 8·53 | 0·13 | |
| 24 | 9·2 | 0·06 | 9·31a | 0·03 | 9·19a | 0·08 | 9·12 | 0·21 | 9·2a,b | 0·07 | 9·24a | 0·04 | 8·25 | 0·07 | |
| Bac303 | 0 | 8·26 | 0·16 | 8·23 | 0·35 | 8·27 | 0·33 | 8·33 | 0·25 | 8·33 | 0·3 | 8·2 | 0·19 | 8·26 | 0·29 |
| 4 | 8·07 | 0·55 | 8·41 | 0·53 | 8·09 | 0·86 | 8·49 | 0·15 | 7·94a | 0·26 | 8·39 | 0·12 | 7·75 | 0·83 | |
| 8 | 7·33 | 0·64 | 7·83 | 0·82 | 8·11 | 0·66 | 8·31 | 0·46 | 7·88 | 0·32 | 8·63 | 0·19 | 6·71a | 0·37 | |
| 24 | 7·78a | 0·05 | 8·39 | 0·15 | 7·06a | 0·2 | 7·94b | 0·03 | 8·41 | 0·06 | 8·29 | 0·04 | 8·39a,c | 0·2 | |
| Erec482 | 0 | 8·35 | 0·16 | 8·34 | 0·13 | 8·42 | 0·03 | 8·34 | 0·06 | 8·38 | 0·09 | 8·3 | 0·04 | 8·32 | 0·05 |
| 4 | 8·29 | 0·12 | 8·39 | 0·15 | 8·17 | 0·31 | 8·4 | 0·21 | 8·36 | 0·2 | 8·24 | 0·34 | 8·08 | 0·43 | |
| 8 | 7·85 | 1·21 | 7·92 | 1·42 | 7·88 | 1·22 | 7·91 | 1·36 | 8·45 | 0·08 | 8·38 | 0·38 | 7·98 | 0·76 | |
| 24 | 8·85a,b | 0·37 | 8·65a | 0·06 | 7·94a | 0·04 | 8·58a | 0·01 | 8·59a | 0·01 | 8·56a | 0·04 | 8·06a | 0·05 | |
| Rrec584 | 0 | 8·34 | 0·25 | 8·24 | 0·08 | 8·06 | 0·54 | 8·15 | 0·3 | 8·17 | 0·17 | 8·28 | 0·09 | 8·34 | 0·04 |
| 4 | 7·54 | 1·04 | 8·13 | 0·3 | 7·85 | 0·59 | 8·2 | 0·41 | 7·29 | 0·86 | 7·77 | 0·62 | 7·78 | 0·69 | |
| 8 | 7·06 | 0·87 | 7·47 | 0·74 | 7·37a | 0·78 | 7·71 | 0·98 | 7·21 | 0·78 | 7·6 | 0·56 | 7·66a | 0·21 | |
| 24 | 7·2 | 0·02 | 7·14a,b | 0·05 | 6·92 | 0·01 | 7·78 | 0·14 | 7·23a | 0·06 | 8·51a | 0·03 | 7·54a | 0·2 | |
| Chis150 | 0 | 5·83 | 0·2 | 5·73 | 1·1 | 5·83 | 0·2 | 5·73 | 1·1 | 5·93 | 0·3 | 5·73 | 1·1 | 5·73 | 1·1 |
| 4 | 5·86 | 0·04 | 6·34 | 0·5 | 5·79 | 0·1 | 6·56 | 0·6 | 6·11 | 0·2 | 6·2 | 0·3 | 5·9 | 0·1 | |
| 8 | 6·03 | 0·1 | 6·74 | 1 | 6·53 | 1 | 6·80 | 1·1 | 6·92 | 1·3 | 7·24 | 0·8 | 5·87 | 0·2 | |
| 24 | 5·73 | 0·02 | 5·73 | 0·05 | 7·45 | 0·01 | 5·73 | 0·2 | 6·43 | 0·03 | 5·73 | 0·01 | 5·73 | 0·01 | |
| Prop853 | 0 | 7·98 | 0·18 | 7·97 | 0·27 | 8·05 | 0·22 | 8·18 | 0·26 | 8·16 | 0·22 | 8·08 | 0·22 | 8·04 | 0·06 |
| 4 | 8·04 | 0·28 | 7·97 | 0·02 | 7·81 | 0·47 | 8·02 | 0·09 | 8·02 | 0·05 | 8·11 | 0·09 | 7·99 | 0·19 | |
| 8 | 7·84 | 0·37 | 7·74 | 0·48 | 7·83 | 0·37 | 7·85a | 0·68 | 7·67a | 1·03 | 8·16a | 0·3 | 7·76a | 0·08 | |
| 24 | 8·19b,c | 0·01 | 7·99 | 0·01 | 7·95a,b | 0·04 | 6·53b | 0·02 | 6·15b | 0·06 | 8·72b,c | 0·01 | 8·1c | 0·07 | |
B-GOS, Bimuno® galacto-oligosaccharide.
a Significantly different from 0 h with P<0·05; b significantly different from 4 h with P<0·05; c significantly different from 8 h with P<0·05; italicised superscript letters were significantly different with P<0·01; bold superscript letters were significantly different with P<0·001 (t test).
*
The 65 % B-GOS and 52 % B-GOS were tested at 1, 0·5 and 0·33 g (equivalent to 0·1, 0·05 and 0·033 g/l).