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Morphology of the apicoplast in ATG8-FRT late schizonts. Double IFA of Hepa 1-6 cells that were infected with either the ATG8-FRT strain or the UIS4-FLP strain for 40 h using antibodies against ACP (red) and PbATG8 (green). Shown are the three optical z-slices of a PV, the extended-focus image, and the image with the positive PDM. PCCs were calculated from 3 independent parasite preparations. Bars, 4 µm.
