FIG 2 .

Distribution of micronemes relative to PbATG8-containing structures in P. berghei-infected Hepa 1-6 cells. Double IFA on PV using antibodies against PbATG8 (green) and TRAP (red) to label micronemes 17 h p.i. (A) Shown are the phase image at low magnification showing the PV in a Hepa 1-6 cell (hnu, host nucleus), the extended-focus image (a merged image displaying the brightest point of all z-slices in a volume, thus providing maximum depth of field for the regions of the image), the merge image plus the positive product of the differences from the mean (PDM), and a 3-D rotated view of a representative PV. (B) Two optical z-slices of a PV and the 3-D reconstructions of the z-stacks of the same PV are shown. PCCs were calculated from 3 independent parasite preparations. Bars, 1 µm (except for the phase image in panel A, for which the bar is 7 µm).