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. 2016 Jun 28;7(3):e00682-16. doi: 10.1128/mBio.00682-16

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Copyright © 2016 Voss et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — Distribution of micronemes relative to PbATG8-containing structures in ATG8-FRT parasites. (A and B) Double IFA of Hepa 1-6 cells that were infected with either the ATG8-FRT strain or the UIS4-FLP strain for 17 h using antibodies against PbATG8 (green) and TRAP (red). (A) Two optical z-slices of a PV, the extended-focus image, and the image with the positive PDM. (B) Two 3-D rotated views of the PV shown in panel A with the extended-focus image and the image with the positive PDM. Bars, 2 µm.