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Morphology of the apicoplast in ATG8-FRT young schizonts. (A and B) Double IFA of Hepa 1-6 cells that were infected with wild-type parasites or the ATG8-FRT or UIS4-FLP strain for 17 h using antibodies against ACP (red) and PbATG8 (green). (A) Optical z-slices of a PV at two magnifications, the extended-focus image, the image Bars, 4 microns with the positive PDM, and the phase image. (B) Two 3-D rotated views of the PV shown in panel A with the extended-focus image are represented.
