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. 2016 Jun 16;5(6):e002860. doi: 10.1161/JAHA.115.002860

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© 2016 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Defining the 9 regions of interest (ROIs) within atherosclerotic lesions. Early aortic fibroatheroma stained by Movat pentachrome with ROIs (boxed areas) selected within the fibrous cap, flanking shoulders, and underlying media and the adventitia. Immunostaining for select inflammatory markers within ROIs was quantitatively assessed with an image processing program (ImageJ; plug‐in Cellcounter). An additional high‐resolution image of an adventitial inflammatory infiltrate at ×200 magnification is shown to illustrate the cellular detail. LFA indicates late fibroatheroma.