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. 2016 Mar 14;44(12):5597–5614. doi: 10.1093/nar/gkw156

Figure 3.

Figure 3.

Plants overexpressing POLA5 display pleiotropic phenotypic alterations. (A) 35S::GFP-POLA5 transgenic lines showing strong phenotypic alterations such as early flowering with small curly leaves (L1, L4) and loss of apical dominance (L4, L5). On top, close-up showing the phenotype of flowers from 35S::GFP-POLA5 with two pistils (left) and mature inflorescences carrying these flowers (right). (B) 35S::POLA5-HA transgenic lines displaying early flowering phenotype. The close-up shows that the inflorescences of these plants display a number of flowers with homeotic alterations. (C and D) Expression analysis of FT, SOC1, SEP3, PI and FLC genes relative to UBCin WT, esd7-1 (Col background) and transgenic lines L1 (C) and L4 (D) grown under LD conditions. Total RNA was extracted from pools of 9-day-old seedlings collected 4 h. Data shown are mean ± SD (n = 3). Asterisks indicate statistically significant differences (P < 0.05) according to Student's t-tests comparing expression levels of these genes in mutants or transgenic plants with the corresponding WT.