Table 1. Identified POLA5-interacting proteins related to chromatin remodeling and gene expression regulation processes.
| POLA5-HA | GFP-POLA5 | |||||
|---|---|---|---|---|---|---|
| AGI locus | Protein | Description | R1 | R2 | R3 | R4 |
| At1G09770 | ATCDC5 | Cell division Cycle 5 protein | 8 | 2 | 10 | 19 |
| At5G03740 | HD2C | Histone deacetylase 2C protein | 7 | 2 | 10 | 10 |
| At5G15020 | SLN2 | SIN3-like 2 protein | 7 | 2 | 10 | 10 |
| At5G58230 | MSI1 | Multicopy suppressor of Ira 1 protein | 1 | 2 | 7 | 18 |
| At4G29830 | VIP3 | Transducin/WD40 repeat-like superfamily protein | 1 | 8 | 6 | 15 |
| At2G46020 | CHR2/BRM | SWI/SNF chromatin remodeling complex BRAHMA protein | 1 | 5 | 6 | 10 |
| At1G80670 | RAE1 | Transducin/WD40 repeat-like superfamily protein | 1 | 1 | 4 | 5 |
| At3G18165 | MOS4 | Modifier of snc1,4 (MOS4) protein | 2 | 12 | 2 | 4 |
| At1G79730 | ELF7 | Hydroxyproline-rich glycoprotein family protein/ EARLY FLOWERING 7 | 1 | 7 | 1 | 2 |
| At4G15900 | PRL1 | Pleiotropic regulatory locus 1 protein | 3 | 1 | 1 | 1 |
| At4G38130 | HDA19 | Histone deacetylase 19 | 2 | 8 | 3 | 2 |
| At4G26630 | DEK3 | DEK domain-containing chromatin associated protein | 2 | 6 | 1 | 1 |
| At2G45640 | SAP18 | SIN3 associated polypeptide P18 | 1 | 4 | 1 | 2 |
| At2G37190 | RPL12A | Ribosomal protein L11 family protein | 2 | 1 | 1 | 1 |
A selection of proteins identified as interactors of POLA5 C-terminal fragment of the catalytic subunit of DNA Pol ϵ related to chromatin remodeling and gene expression regulation processes. These chromatin remodeling proteins, transcriptional and translational regulators were pull-down by POLA5 and detected by mass spectrometry analyses in four independent experiments. No peptides from these proteins were identified in the corresponding negative control of the pull-down assays with the exception of one peptide in the sample R1 for HDA19.